Human CD11a (alpha L integrin) complexes with CD18 (beta 2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM’s 1, 2, and 3, also designated CD54, CD102, and CD50 respectively. LFA-1 is expressed on lymphocytes, monocytes and neutrophils and plays a role in cell-cell adhesion (6).

Isotype: Murine IgG2a

Immunogen: Human monocytes

Specificity: Antibody 38 recognizes the I domain of the human integrin alpha L subunit (CD11a) of about 180 kd (1, 3, 7).

Functional Application: Antibody 38 blocks binding of ICAM-1 and ICAM-3 to LFA-1 at 5-10 ug/ml (7, 9).

References:

1. I. Dransfield & N. Hogg, (1989) EMBO J 12: 3759-3765.

2. I. Dransfield, et al, (1992) J Cell Biol 116: 1527-1535.

3. R.C. Landis, et al, (1994) J Cell Biol 126: 529-537.

4. A.M. Randi & N. Hogg, (1994) JBC 269:12395-12398.

5. P. Stanley, et al, (1994) EMBO J 13:1790-1798

6. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1581-1592.

7. C.L. Holness, et al, (1995) J Biol Chem 270: 877-884.

8. M. Galdiero, et al. Microbiology 147 (2001), 2697-2704

9. T Mizuno, K Mori, et al (1996) J Biol Chem 272(2): 1156-63. PMID: 8995416