Molecular Structure: A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker: (1)kpqapelrgs(10)
A CD70-reactive n-terminal section of the mature extracellular domain of human CD27:
(11)kscperhywaqgklccqmcepgtflvkdcdqhrkaaqcdpcipgvsfspdhhtrphcescrhcnsgllvrnctitanaecacrngwqcrdkectecdplpnps (113)
linker (114)gt(115)
murine IgG2a Fc + hinge regions: (116) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk (348)
The molecule is dimeric with a predicted monomeric non glycosylated molecular weight of 39.3 kd.
Transfectant Cell Line: CHO
Human CD27 is a lymphocyte specific member of the tumor necrosis factor receptor family (TNFRSF7) and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily(TNFSF7). The CD27-CD70 interaction plays an important role in T cell activation.
Specificity: Recombinant soluble CD27-muIg binds to cell surface CD70 on Raji cells in FACS, and is reactive with recombinant CD70-muCD8 (cat #537-020), and anti-CD27 mAb clone M-T271 (cat #176-020).
