Human CD47 (IAP, Integrin Associated Protein) is a broadly expressed Ig superfamily glycoprotein of about 50kD with a long extracellular Ig like domain, 5 transmembrane domains and an alternatively spliced intracellular c-terminal domain. It interacts with it ligand SIRP alpha (CD172a, Signal Regulatory Protein) expressed mainly on myeloid cells serving as a negative immune regulator, inhibiting phagocytosis. The CD47-SIRPa axis is being investigated as possible anti-tumor immune intervention point. Blockade of this interaction has shown promise in models of HNSCC(2), Glioma(3) and Breast Cancer(4)

Isotype: Mouse IgG1k

Immunogen: Recombinant human CD47 (extracellular Ig domain)

Specificity: Clone ANC2F6 binds to an epitope within the extracellular Ig-like domain of human CD47.

References:

1) Xiaojuan Liu, Yang-xin Fu, et al. (2017) J Hematol Oncol 10:12. doi:10.1186/s13045-016-0381-z PMID: 28077173.

2) Wu L, Sun ZJ, et al. (2018) Oncoimmunology 7(4): e1397248. Doi: 10.1080/2162402X.2017.1397248.

3)Lif F, Jiang X, et al. (2017) Oncoimmunology 7(2): e1391973. doi: 10.1080/2162402X.2017.1391973.

4) Norman MZ, Janji B, et al. (2018) Oncoimmunology. 7(4):e1345415. doi: 10.1080/2162402X.2017.1345415.