Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (5).

Isotype: Murine IgG1

Immunogen: Murine L cells transfectants expressing human CD40

Specificity: Antibody EA-5 recognizes the CD40 molecule of about 44 kd (1, 2, 3).

Functional Application: Binding of cross linked EA-5 will partially activate B cells.

References:

1. C.L. Law, B. Wormann & T.W. LeBien, (1990) Leukemia 4: 732-738.

2. A.W. Larson & T.W. LeBien, (1994) J Immunol 153: 584-594.

3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 547-556.

4. E. Lindout, et al, (1995) J Exp med 181:1985-1995.

5. T.M. Foy, R.J Noelle et al, (1996) Annu Rev Immunol 14:591-617.