Human CD252 (CD134L, OX40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 binds to CD134(OX40) present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8).
Isotype: Murine IgG1 Kappa
Immunogen: Human HUVEC and Recombinant soluble human CD252
Specificity: Antibody ANC10G1 binds to CD252 on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.
Functional Application: Antibody ANC10G1 blocks binding of Recombinant CD134 to CD134L on HUVEC.
References:
1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763.
2)L.M. Higgins, et al, (1999) J Immunol 162: 486-493.
3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.
4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.
5) V. Malmstrom, et al, (2001) J Immunol 166: 6972.
6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115.
7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955.
8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.
9) T Uenotsuchi, M Furue, et al. (2006) Intl Immunol 18(12): 1637-1646.
