Human CD32 exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C) . The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses.
Isotype: Murine IgG1k
Immunogen: Human K562 tumor cells and L cells transfected with human Fc gamma RII
Specificity: Antibody 7.3 reacts with a domain 2 epitope of all CD32 isoforms (1). It exhibits preferential binding to Fc gRIIb (inhibitory) isoform transfectants(2, 5).
Functional Application: Intact and F(ab‘)2 7.3 antibody blocks immune complex binding (4). Incubation with anti-CD32 F(ab‘)2 inhibits Fibrocyte differentiation(6), and inhibits pro inflammatory cytokine secretion by SLE patient derived monocytes (7).
References:
1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 823-836.
2. Liu Y, L Pricop, et al. (2005) J Leukocyte Biol 77: 767-776 PMID:15703199
3. S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425
4. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.
5. Ierino FL, P M Hogarth, et al. (1993) JImmunol 150(5):1794-1803.
6. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.
7. Thanei S, M Trendelenburg (2016) J Immunology 196: 2063–2074. PMID: 26829984
