Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP (5).

Isotype: Murine IgG2a

Immunogen: Human thymocytes/Sezary T cells

Specificity: Antibody UCHM1 recognizes an extracellular epiotpe of the human CD14 molecule of about 53 kd. UCHM1 will also immunoprecipitate a soluble form of CD14 (1, 2, 3). It is crossreactive with cynomolgus monkey CD14 (6).

References:

1. N. Hogg, et al, (1984) Immunology 53: 753-767.

2. M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151.

3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989)”Reference Antibody”.

4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788.

5. S.D. Wright, (1995) J Immunol 155: 6-8.

6. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.