Human CD106 is an endothelial adhesion molecule that binds to alpha 4 beta 1 & alpha 4 beta 7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells (3).

Isotype: Murine IgG1

Immunogen: Human endothelial cells

Specificity: Antibody 1.G11B1 recognizes the an extracellular epitope of the human VCAM-1 molecule (CD106) which is involved with leukocyte adhesion (1, 2,3). It has been reported to be cross reactive with porcine CD106.

Functional Application: Antibody 1.G11B1 binds to an epitope located within the first three n-terminal loops of CD106. This mAb blocks lymphocyte(3,12) and eosinophil (6) adhesion to CD106. It has been reported to work in FACS(10, 11), IHC (frozen section) and Western blot(8).

References:

1)S M Wellicome SM, Haskard DO et al. (1990) J Immunol 144(7):2558-65.

2) M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598.

3) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767.

4) M. Nagata, et al, (1995) J Immunol 155: 2194-2202.

5) H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-3144.

6) K.D. Patel (1998) Blood 92 (10): 3904-3911.

7) S.R. Barthel, M.W. Johansson, et al. (2006) J Biol Chem 281(43): 32175-32187.

8) H Valentin, L Gazzolo, et al. (1997) J Virol 71(11): 8522-8530.

9) A Maenpaa, T Timonen, et al. (1997) ACTA Neuropathologica 94(3):216-225.

10) H Yarwood, D O Haskard, et al. (2000) J Leukoc Biol 68: 233–242.

11) AF Tramontano, L Ragolia, et al. (2010) Mediators of Inflammation 2010: 250476 doi:10.1155/2010/250476.

12) XY Zhou, ZC Han, et al.(2017) Exp Ther Med 14(4): 3541-48. doi.org/10.3892/etm.2017.4952