Human CD105 (endoglin) is a homodimeric membrane glycoprotein (1) virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity (4).

Isotype: Murine IgG1 kappa

Immunogen: Membrane preparation of human B-lineage leukemia cells

Specificity: Antibody SN6 recognizes an extracellular epitope of the human CD105 homodimer with a molecular weight of about 160 kd. It has been reported to cross react with non human Primate as well as Equine and Porcine(11) CD105.

Functional Application: Antibody SN6 will augment binding of TGF-b1 to CD105 expressing leukemia cells. Antibody SN6 has been used to identify mesenchymal progenitor cells populations (8,9,10).

References:

1. Y. Haruta & B.K. Seon, (1986) Proc Natl Acad Sci USA 83: 7898-7902.

2. H. Hara, et al, (1988) Cancer Res 48: 4673-4680.

3. W.C. Biddle, et al, (1989) Leukemia Res 13: 699-707.

4. S. Cheifetz, et al, (1992) J Biol Chem 267: 19027-19030.

5. B.K. Seon, et al, (1995) Proc Am Assoc Can Res 36: 23.

6. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1756-1761, 1762-1764.

7. B.K. Seon, et al, (1996) Tissue Antigens 48: 417.

8. S Alsalameh, M Lotz, et al. (2004) Arthritis & Rhematism 50(5): 1522-1532.

9. A Banas, T Ochiya, et al. (2007) Hepatology 46: 219-228.

10. R Williams, HE McCathy, et al. (2010) PLoS ONE 5(10): e13246 doi:10.1371.

11. G Vilahur, L Badimon, et al. Oct 13, 2014 Toxicol Pathol pii: 0192623314551971.

12. M Vigano, de Girolamo, et al. (Feb 2017) J Exp Orthopaedics 4: 8.